Cell lineage analysis by intracellular injection of fluorescent tracers.
نویسندگان
چکیده
Cell lineages during development of the leech are revealed by injection of a fluorescent peptide, rhodamine-D-peptide, into identified embryonic cells. Use of this peptide together with a nuclear stain showed a stereotypic cleavage pattern of stem cells and their progeny. Combined injection of rhodamine-D-peptide and pronase demonstrated the arrest of stem cell production in the pronase-injected teloblast.
منابع مشابه
Cell-lineage and clonal-contribution map of the trochophore larva of Patella vulgata (Mollusca)1Both authors contributed equally to this work.1
Molluscan development is characterised by its extremely regular cleavage pattern. In numerous molluscs the fate of various early-cleavage stage blastomeres has been determined and fate maps have been constructed. On the basis of similarities between these fate maps, a generalised molluscan cell-lineage map has been constructed. Recently, the validity of this map has been challenged. In this stu...
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ورودعنوان ژورنال:
- Science
دوره 209 4464 شماره
صفحات -
تاریخ انتشار 1980